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Cell Cycle Synchronization : Methods and Protocols
To prepare synchronized cells representing different stages of the cell cycle has been a great challenge for researchers across the globe. In Cell Cycle Synchronization: Methods and Protocols, experts in the field supply detailed protocols providing first the theoretical background of the procedure then step-by-step instructions on how to implement synchronization as well as the latest techniques for the enhanced study of regulatory mechanisms to understand cell cycle events. Describing synchronized cells from asynchronous bacterial, plant, protozoan, yeast, fish, and mammalian cell cultures, the synchronization methods presented in the book are based principally on two major strategies: the “arrest-and-release” approach, which involves different chemical treatments to block cells at certain stages of the cell cycle, and the physical strategy, which contains physical methods to collect cells belonging to subpopulations of the cell cycle. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Detailed and easy to follow, Cell Cycle Synchronization: Methods and Protocols is an ideal guide for scientists who wish to make use of these powerful synchronization techniques but have no access to thorough, time-tested protocols designed for doing so.
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Work–Family Triangle Synchronization : Employee, manager, and spouse
"I need to check with my partner" is a common response of an employee to his manager, emphasizing the tug of war between the employee’s spouse and the workplace. The challenges in the fields of work and family have been the focus of researchers for decades.Frameworks for work–family conflict, work–family enrichment, and work–family balance have been put forth in light of the complexity of the interface.Yet the relationship between the three stakeholders managing the interface (manager, employee, and spouse), has not received the attention it deserves. Work–Family Triangle Synchronization takes a holistic look into the triangle of forces involved in the conflict: the manager, the employee, and the employee’s spouse at home.Using the therapeutic triangle relationship framework, it elaborates on the dynamic of work–family triangles and offers a structured process for designing a psychological contract among the three players.This process is termed work–family triangle synchronization (WFTS). Based on the authors’ 20 years of academic research and field experience in the organizational and family domains this book introduces a novel synchronization model, methodology, and compelling tools.Personal anecdotes and stories make the text accessible and understandable, accompanying the reader step by step in the task of developing a synchronized work–family triangle psychological contract, as both a diagnostic and a management tool.
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David LaChapelle. Good News
TASCHEN presents Lost + Found and Good News, the latest and final publications from artist David LaChapelle.The books are the fourth and fifth installments of LaChapelle’s five-book anthology, which began with LaChapelle Land (1996), continued with Hotel LaChapelle (1999), and followed by Heaven to Hell (2006). Good News follows David LaChapelle’s creative renaissance as he surrenders to contemplations of mortality, moving beyond the material world in a quest for paradise.Featuring a monumental curation of images, it is a sublime and arresting new body of work that attempts to photograph that which can’t be photographed.It represents the final chapter to LaChapelle’s narrative in a collection of books that have captivated a generation of viewers across the globe. Good News features: Pamela Anderson, Lana Del Rey, Sharon Gault, Janet Jackson, Michael Jackson, Paris Jackson, David LaChapelle, Amanda Lepore, Miriam Makeba, Sergei Polunin, Tupac Shakur, Elizabeth Taylor, and many more... Lost + Found and Good News are sold separately.
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